FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10664269
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC138400.12977917192448915No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG122830.11517901508298412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA57250.016.32182768
TGCGCAT61050.015.30617669
GGTGCGC63450.014.72708167
CGGGGGT8800.013.9250771
AGGTGCG67550.013.886126566
CGGGCAT14300.013.7108461
CCGGGAT26400.012.7314991
CGGGGGG12050.012.2032211
GCGGGCT12150.012.1027821
CGGGAAT64900.011.7604531
GACTCTA43150.011.75952154
CGGCAAT20300.011.555561
ACTCTAG41800.011.5532795
CGAGCGC37750.011.50163959
GCGCGCC38450.011.47330462
AGCGCGC38500.011.27705361
AAGGTGC85900.011.08290365
CCGGGGT8250.011.0339671
CTAGCAG33950.011.0285898
GCTGACT33600.010.836931
Produced by FastQC (version 0.11.2)