FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10514884
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC151450.1440339237218404No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG133690.1271435804712634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT8900.016.9156151
GTGCGCA63150.014.74172268
TGCGCAT67050.014.04098569
GGTGCGC68000.013.79329167
GGGGCAC6450.013.5703061
GCTGACT31450.013.47020151
CGAGCGC40400.013.17542459
AGGTGCG72600.013.01723566
AGGGGGG6400.012.5764144
AGCGCGC42700.012.30130861
CGCGGGG8300.012.2328961
GCGCGCC43050.012.11943562
CTAGCAG31800.012.1036028
ACTCTAG42650.011.8975615
GACTCTA44900.011.4572394
TCTAGCA68400.011.4075317
ACGAGCG48400.011.07002158
GCGCCGA48850.010.9636164
CGGGAAT73550.010.7104871
CCGGGAT25600.010.6675331
Produced by FastQC (version 0.11.2)