FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11821065
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC181630.15364943852351712No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG162320.1373141929259335No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG122370.1035185915989803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA72900.015.84206568
TGCGCAT77750.015.03389369
GGTGCGC79600.014.72851567
AGGTGCG87400.013.45547966
CCGGGGT8450.013.26104451
CTAGCAG37000.012.9564328
ACTCTAG46600.012.9175145
CGGGGGT11650.012.6243161
GCTGACT37150.012.5365291
CGGGCAT15450.011.7858071
GACTCTA47400.011.7396514
TCTAGCA80300.011.5912167
AAGGTGC106800.011.30645665
GGGGCAT12800.011.2165261
GGGGCCT9750.011.1337531
GTCGGGA91950.010.9679441
GGGCCTC11500.010.9560952
AGCGCGC48350.010.78980561
CTGTACG118950.010.7096026
TGTACGA123000.010.6681487
Produced by FastQC (version 0.11.2)