FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9359318
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC129690.138567788806834No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG117440.12547922829419836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA50400.016.38788268
TGCGCAT54450.015.169353569
GGTGCGC56350.014.71967167
GCTGACT30600.013.9598811
AGGTGCG60050.013.87240366
CTAGCAG30000.013.7639098
CGGGCAT10600.013.2128781
CGGGCGC7800.013.01049215
ACTCTAG39650.012.8862885
GACTCTA39150.012.7826274
CCGGGGT6250.012.3249731
CGAGCGC33450.012.03774159
AGCGCGC34850.011.85512961
TCTAGCA63950.011.76452357
GCCGAAT15850.011.7081941
GCGCGCC36250.011.59007862
TCGGGCG10350.011.4951587
CTGTACG90150.011.2577196
CGGGGGG10000.011.2045211
CGGGGGT9100.011.1583481
Produced by FastQC (version 0.11.2)