FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13328480
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC138890.10420543077680276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCA55800.016.55804468
CCGGGGT8750.016.0075911
TGCGCAT61450.015.14975569
GGTGCGC67650.014.07208267
AGGTGCG69200.013.4541866
TAGCCCG18450.013.2766634
CGGCAAT30000.013.0728661
CGGGCGC19550.012.35101115
GGGGCAC8300.012.2347171
GGCGGGC21550.011.69186513
CTAGCAG40400.011.5197438
CGGGAAT73450.011.25108051
GGCAGAC24450.011.1709411
TCGGGCG27800.011.0766587
CGGGGAG27050.011.0033681
CGAGCGC39450.010.8281159
TCTAGCA84150.010.81160457
GCTGACT45600.010.7507111
CTGGGGG7550.010.6673111
CGGGCAT20200.010.5743211
Produced by FastQC (version 0.11.2)