FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_pchx_d2_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_pchx_d2_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12598210
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC172410.13685277511646496No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG152180.12079493832854032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT9800.017.151011
GTGCGCA68000.016.98379368
TGCGCAT72400.016.00022169
GGTGCGC80150.014.977267
AGGTGCG79600.014.5980966
TAGCCCG17750.014.1945234
CGGGCGC17750.013.60389215
CGGCAAT29750.012.9473311
CTAGCAG38250.012.6251338
CGAGCGC48350.012.0932959
TCGGGCG22150.012.0066827
GGCGGGC20350.011.865849513
CGGGGGT14500.011.5917181
AGGGGGG7350.011.4280614
GCTGACT41750.011.406621
AAGGTGC108000.011.37503465
CGCGTAT14750.011.15784551
GGCGCAT22050.011.109467517
ACGAGCG53350.011.02565958
ACTCTAG56450.010.9738665
Produced by FastQC (version 0.11.2)