FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12423194
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC365380.2941111601412648No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG296850.23894821251282078No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG271610.2186313761179291No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT177660.143006701819194No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA177050.14251568477478496No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG176250.14187172799523215No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG173520.13967422548500813No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG164400.1323331181981059No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164320.13226872252015062No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC163280.13143157870673194No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG160380.12909723538085294No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC147420.11866513555209715No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG147060.11837535500129838No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT139960.11266023858276704No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA135790.10930361386934793No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG128070.10308943094666315No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC127360.10251791930481002No Hit
CTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAA125260.10082753275848384No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA124330.10007893300225368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG61900.018.6014845
CTAGCAG53550.017.839218
GACTCTA61800.017.8385354
GCTGACT54200.017.573371
CTGACTC55300.017.4045942
CCGGGGT11400.016.894421
GTGCGCA133500.016.33210668
TGCGCAT138850.015.77906669
AGGTGCG141700.015.33938166
GGTGCGC145700.015.22908667
CGCGGGG11400.014.7442221
CTCTAGC72000.014.2908216
TAGCAGA69750.013.8465259
TCTAGCA119900.013.6290837
TCTATCG72600.012.96598616
AAGGTGC172500.012.84390865
CTATCGA73750.012.43161217
GTCGGGA191150.012.2739951
CGGCAAT24700.012.1923361
CGAGCGC100800.012.01854959
Produced by FastQC (version 0.11.2)