FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17612154
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC529090.30041186330757724No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG434720.2468295473682549No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG289770.1645284273576077No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC244990.1391028036661501No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA239100.13575852221142287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234450.1331183000103224No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG234360.1330671989354624No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG222120.12611745275450123No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG213170.12103573475453372No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC210990.11979795316348017No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT209960.1192131297511934No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG208120.11816839666516656No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT206350.11716340885958638No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC180840.10267909308537729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG68500.022.5271918
CTGACTC75200.021.0364342
ACTCTAG93150.019.3109955
GCTGACT81700.019.1152611
GACTCTA96700.018.602064
GTGCGCA191600.016.89562668
TAGCAGA95100.016.8886019
TGCGCAT195650.016.54607269
CTCTAGC102100.016.487086
AGGTGCG204500.015.91703366
GGTGCGC213000.015.44480867
TCTAGCA171200.014.53197
TCTATCG106450.014.3983316
CCGGGGT14450.014.2972191
CTATCGA107800.014.18551617
AGATCTA118850.013.27890613
TAGCCCG26950.013.24399234
AAGGTGC251950.013.04446365
CGAGCGC122250.013.03223159
ACGAGCG125900.012.7936958
Produced by FastQC (version 0.11.2)