FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15111952
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC438020.2898500471679635No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG386260.2555990119608638No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG249960.1654055015526783No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA234040.15487079366054102No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC211810.14016058282874375No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG203580.1347145623543537No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG197490.13068463954888157No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT193090.12777303686512503No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG187370.12398795337624152No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG186400.12334607733004975No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC176090.11652366285970205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175620.11621265075484623No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT169560.11220257978585427No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC161930.10715359604106738No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG161400.10680288026325123No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG159690.1056713255838822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG61000.022.4288868
CTGACTC64000.021.2714212
GCTGACT72700.019.313441
GACTCTA89300.017.9089494
ACTCTAG88000.017.8952625
TAGCAGA81450.017.2701939
GTGCGCA168550.016.17499768
TGCGCAT172200.015.85273369
CTCTAGC92250.015.5154776
AGGTGCG177250.015.42214966
TCTAGCA150900.015.0955267
CGGCAAT37950.014.8546981
GGTGCGC188900.014.7106367
CCGGGGT11700.014.3649831
TCTATCG93750.014.18396216
CTATCGA99150.013.41132717
GACTGTC36400.013.2673154
TAGCCCG28250.013.25393334
AGATCTA102850.013.23528813
CGAGCGC104200.012.80336959
Produced by FastQC (version 0.11.2)