FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13053515
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC337540.2585816923640874No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG285890.21901380585995417No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG199430.15277877261411965No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA178430.136691151770232No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT155550.1191633058222249No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG150870.11557806460558709No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG150310.11514906138308341No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG149410.11445959191834537No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC149010.11415316104512847No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG147310.1128508298339566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC52350.018.2513162
CTAGCAG53150.018.0389258
ACTCTAG66350.017.3006655
GCTGACT58050.016.7079121
GACTCTA68950.016.3436184
TATGCCG16950.016.1111448
GTGCGCA129050.015.45818268
CGTATGC16550.015.43919646
TGCGCAT131350.015.2676769
CCGGGGT10100.015.2537241
GTATGCC18050.014.73933147
TAGCAGA69100.014.4321229
AGGTGCG138400.014.41507666
GGTGCGC144350.013.91687267
TCTAGCA119050.013.7850027
GGCAGAC22100.013.7838841
TCTATCG69650.013.71586516
CGTAGCG19800.013.07810725
CTCTAGC79550.012.8462366
CTATCGA74050.012.57003817
Produced by FastQC (version 0.11.2)