FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13216852
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC405530.3068279799153384No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG358830.271494301366165No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG245980.18611088328748782No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA229740.17382353982627632No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG182960.13842933249157968No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT180160.13631082499826738No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC178910.13536506272446722No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG178070.13472951047647352No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG177640.13440416825428628No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG176300.13339031109677252No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC163470.12368300711848783No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT156900.11871208060739424No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG152620.11547379058190256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150420.1138092489800143No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC142230.10761261456207576No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG142080.10749912308921973No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT136960.10362528081573434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC59300.021.8370862
CTAGCAG59400.021.737628
GCTGACT60250.021.329481
ACTCTAG70900.020.4845545
GACTCTA69950.020.0623264
CCGGGGT10400.018.8549731
TAGCAGA77500.017.0671969
GTGCGCA152400.016.94776768
TGCGCAT156750.016.49983669
CTCTAGC82400.016.3939936
AGGTGCG162200.015.88220466
TCTATCG80600.015.54323716
GGTGCGC167900.015.48771967
CTATCGA84750.014.823317
TCTAGCA142950.014.7604737
AAGGTGC195150.013.45183365
TGACTCT109350.013.4097263
GATCTAT94950.013.30481914
ATCTATC95250.013.29955215
AGATCTA96900.013.07308913
Produced by FastQC (version 0.11.2)