FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14459998
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC382930.264820230265592No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG354340.24504844329853986No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG248090.1715698715864276No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA227690.15746198581770204No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT185510.12829185730177833No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG179970.1244605981273303No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC175000.12102352987877316No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG172800.11950209121744No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG166580.11520056918403446No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC160300.11085755336895621No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT155210.1073374975570536No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG154810.10706087234590213No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG147720.10215769047824211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG59700.019.8110778
CTGACTC62000.019.4745182
GCTGACT67000.017.8728941
ACTCTAG78550.017.1975615
GACTCTA83600.016.8284474
GTGCGCA145900.016.14377468
TGCGCAT150600.015.59374169
CCGGGGT10750.015.3085141
AGGTGCG155250.015.28554466
TAGCAGA80500.015.0399339
TCTAGCA144500.014.9166937
GGTGCGC162550.014.85654967
CGGCAAT35050.014.4851691
CTCTAGC87700.014.3657636
TCTATCG89200.013.18143316
CGAGCGC93850.012.83465959
CGCGGGG10300.012.57791
ACGAGCG99000.012.37942658
CTATCGA93250.012.34617117
TAGCCCG24050.012.2219934
Produced by FastQC (version 0.11.2)