FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13849626
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC401760.2900872557858241No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG349760.2525411155507015No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG262330.18941305707460981No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA236050.17043781543270556No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC180550.13036453114329585No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT179810.12983022068610373No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG177970.12850166495470708No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG177370.12806844025968642No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG161700.11675405530806392No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG160770.11608255703078191No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC155680.11240736753469009No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT154810.11177919172691017No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG150040.10833505540149604No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC139310.10058755377221017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG54400.021.4840988
CTGACTC58950.020.1849372
GCTGACT66700.017.7978741
ACTCTAG78700.017.5204125
GACTCTA80750.017.5088884
GTGCGCA141400.017.49912868
CCGGGGT9500.017.324811
TGCGCAT144400.017.1844869
AGGTGCG152800.016.26386566
TAGCAGA74100.016.0557969
TAGCCCG29250.016.03136334
GGTGCGC163150.015.65971267
CGGCAAT37100.015.3853691
CGGGCGC31400.015.04533815
CCCTACA12650.014.93743110
CTCTAGC84350.014.9362066
TCGGGCG35700.014.6044257
GGCGGGC32200.014.56270413
TCTAGCA138350.014.4924547
GGCGCAT32800.014.18967417
Produced by FastQC (version 0.11.2)