FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14790807
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC459320.31054424548978293No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG398460.26939706535282354No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG302000.20418088073220075No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA262340.17736692798438922No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT221070.14946446126976032No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG206860.13985714234524188No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG200900.13582761238112295No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC199570.1349284051911434No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG194960.1318116043296353No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG193080.13054054454229574No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC181740.12287362008036479No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG178470.12066278736515187No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT169440.11455764381213275No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT168660.11403028921951318No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTG160330.10839841260858857No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC159770.10801979905491296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156320.10568726912601861No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA152620.10318571528923337No Hit
CTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAA149680.10119799413243645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC64450.020.5265372
GCTGACT63900.020.383551
GACTCTA74100.019.9310114
CTAGCAG65350.019.8123238
ACTCTAG71750.019.4620025
CCGGGGT11650.018.3333441
GTGCGCA169100.016.59793568
TGCGCAT175300.016.13106569
GGTGCGC182350.015.44977867
AGGTGCG181700.015.4099466
CTCTAGC88850.015.2831816
TAGCAGA86850.015.0285919
TCTAGCA155600.014.8426747
TCTATCG89250.013.88003216
TGACTCT111450.013.6911983
CGAGCGC109900.013.60422559
CGGCAAT31050.013.5318571
CTATCGA91900.013.47974617
TAGCCCG15200.013.35294134
ACGAGCG113500.013.04950458
Produced by FastQC (version 0.11.2)