FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16503304
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC445560.2699823017257635No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG401680.2433936865005941No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG295880.17928531159578712No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA249650.1512727390830345No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT214850.1301860524413778No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG205810.12470836142871755No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG204190.12372673980919215No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC199520.12089700341216524No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG191570.11607978620523501No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC188840.11442557199455333No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG180520.1093841572572377No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT174660.10583335312734952No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG171490.10391252563729056No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT170250.10316116094086372No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169990.1030036167303226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG65700.020.3455188
CTGACTC72600.019.184892
GCTGACT75500.018.3184221
ACTCTAG93150.017.5444725
GTGCGCA169250.017.41079568
GACTCTA94150.017.2092914
TGCGCAT173300.016.98407469
CCGGGGT11800.016.9133951
AGGTGCG180300.016.36479266
GGTGCGC191100.015.87830667
TAGCAGA88200.015.7902089
CGCGTAT16500.015.2787031
TCTAGCA163650.014.6251057
CGGCAAT42900.013.9565081
GGCAGAC26450.013.76719951
CTCTAGC109300.013.4152526
TAGCCCG32850.013.20876834
AAGGTGC226800.013.13315665
TCTATCG106700.012.59331816
GTGTGCG23300.012.4650188
Produced by FastQC (version 0.11.2)