FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11945468
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA375740.31454606885222075No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC233840.19575624831107497No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG179860.1505675625266419No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG166080.13903180687437278No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135090.11308891372024939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19300.019.58628746
GTATGCC20150.018.58885447
TATGCCG21200.018.00113948
ACTCTAG64150.017.2390165
CTAGCAG39700.016.306398
GCTGACT43000.015.2283071
CTGACTC45700.015.0864972
TCGTATG26200.014.69402245
CTCGTAT28050.013.84897544
GACTCTA52500.013.3985814
GTGCGCA99850.012.44260868
AGGTGCG103850.012.20052966
TGCGCAT103650.012.12165169
ACGTCTG38800.012.0855915
TCTAGCA89150.012.0500327
TCTATCG52950.012.02813716
GCACACG39800.011.9573811
CTCTAGC62350.011.50646
ATGCCGT34350.011.41612749
TATCTCG34300.011.12141141
Produced by FastQC (version 0.11.2)