FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12221358
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC326830.267425273034306No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG251220.2055581711950505No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG231930.18977432786111004No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG150400.12306324714487539No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA150280.12296505838385555No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG148660.1216395101100876No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT145280.11887386000802858No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC142110.11628004023775426No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG139440.11409534030506266No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG135650.1109942119361858No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG124050.10150263170426724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG45500.017.4571638
CCGGGGT14600.017.2685991
CTGACTC49300.016.1854062
ACTCTAG60450.015.9782375
GCTGACT52850.015.7029321
TAGCCCG19400.015.69265134
GTGCGCA119600.015.33324168
GACTCTA60850.015.2980254
TGCGCAT122300.015.13787569
AGGTGCG124750.014.67369366
GGTGCGC132200.014.13690667
TCTAGCA106700.013.8390827
CGGCAAT30950.013.6899581
TAGCAGA63200.012.95566759
CGCTTCA206600.012.78946269
GCGCTTC205900.012.74790468
GGCGCAT24450.012.73751217
TCTATCG65100.012.41670116
CGGGTGT11400.012.2866231
CATGCGT23500.012.20990621
Produced by FastQC (version 0.11.2)