FastQCFastQC Report
Tue 8 Nov 2016
HHCMGBGXY_n01_hho5_mchx_d3_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCMGBGXY_n01_hho5_mchx_d3_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11222557
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC314190.2799629353631262No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG252600.22508239432421684No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG229780.20474834745771395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158860.14155419304174618No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA153390.1366800810189692No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG151600.13508507909561074No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG146760.13077233646485378No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT144790.12901694328663244No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG139910.12466855815479484No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC138760.12364383624872656No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG132130.11773609169461113No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG125240.11159667088347157No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT119330.1063304913488076No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC118250.1053681438196304No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC113100.10077917180549852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG46300.018.8186448
ACTCTAG53750.018.8175415
CTGACTC46700.018.4367732
GACTCTA52150.018.052594
GCTGACT47200.017.952621
CCGGGGT10600.017.1771831
GTGCGCA120550.015.09627868
TGCGCAT122900.014.80768669
CTCTAGC60400.014.7757196
AGGTGCG126850.014.3202166
TCTAGCA102450.014.1060727
GGTGCGC130850.014.04181567
TAGCAGA62150.013.9630579
CGGGGAC13600.013.9030251
TCTATCG62500.013.54948316
CTATCGA66450.012.90198517
CCGGGAT21000.012.6721051
TAGCCCG13900.012.58753334
CGGGGGT15300.012.3582451
AAGGTGC154450.011.94249865
Produced by FastQC (version 0.11.2)