FastQCFastQC Report
Tue 22 Sep 2020
HHCCKAFX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCCKAFX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186311746
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7811560.41927361895905374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA4525810.2429159780403754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG2758060.14803468161368633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG7310300.067.534822
AAGCAGT7382600.067.091671
CAGTGGT7883500.062.437224
AGTGGTA8041550.061.3290025
GCAGTGG8141450.060.584773
GTGGTAT8326250.059.173236
GGTATCA8407750.058.515858
TGGTATC8494700.057.889917
GTATCAA9144850.053.750329
CATGGGG5995500.032.2276424-25
AGAGTAC8445550.032.0097618-19
TACATGG8388850.031.7530722-23
GCAGAGT8561850.031.65131416-17
ACGCAGA8798950.030.8459514-15
CAACGCA8795400.030.80579412-13
ATCAACG8832700.030.7286310-11
AGTACAT9229250.029.15077220-21
ACATGGG8322350.029.02015924-25
CAGAGTA8557050.028.78532818-19
CGCAGAG8601400.028.75227216-17
Produced by FastQC (version 0.11.5)