FastQCFastQC Report
Tue 22 Sep 2020
HHCCKAFX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCCKAFX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186311746
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCGTC4837237925.963139758241542No Hit
CTAAACGG4441779623.84057739440647No Hit
GGTTTACT3933564721.112811105318073No Hit
AACCGTAA3804752320.421430112087513No Hit
GGGGGGGG86132674.623040245675117No Hit
CTAAACGA2872730.15418941970518596No Hit
CTAACGGA2375950.12752550770470478No Hit
TAAACGGA2309890.1239798375353103No Hit
GGTTACTA2108800.11318663719677663No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)