FastQCFastQC Report
Tue 22 Sep 2020
HHCCKAFX2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHCCKAFX2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186311746
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG366200.018.3770471
CTGGACG421450.018.1975671
CGAGGGA239100.017.7096446
CGGCGAG149200.017.6226333
AGGGAAG346350.017.4380873
GCGAGAC241700.017.3916955
GACCGTG328550.017.264491
GAGGGAG212500.017.1889577
ACAGGGA515350.016.8056091
GTGGGAC320300.016.7469068
CGAGACG193050.016.7255276
CTACAGA455750.016.690391
CCGGACA328300.016.6006091
CGAGACC239900.016.5270756
GCGGATC296850.016.3466241
CTACGAG305250.016.3374212
TGCGACG279550.016.334851
ATACCGA461050.016.2980521
GTCCCTG620500.016.1726918
CCCGGAA256150.016.0873321
Produced by FastQC (version 0.11.5)