FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14562950
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT311390.21382343549898888No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC290370.1993895467607868No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG221840.1523317734387607No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC177950.12219364895162037No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG169840.11662472232617704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT70300.034.9643551
CTAGCAG87950.027.7672988
GACTCTA92750.026.9352324
CTGACTC90850.026.883532
ACTCTAG93250.026.6031975
TAGCAGA109800.022.3053879
CTCTAGC112200.022.1100546
CTATCGA112550.021.7617317
TCTATCG113000.021.61291716
TGACTCT117600.021.3627913
TAGCATG121850.019.53031527
CTAGCAT127050.018.89481226
ATTCTAG129350.018.5843623
GATCTAT134250.018.13981614
GCAGATC136600.017.92958811
GCATGAC134300.017.87670329
ATCTATC135200.017.77931215
TTCTAGC136450.017.71992324
AGCAGAT139750.017.62553610
TATCGAT143600.017.08076918
Produced by FastQC (version 0.11.2)