FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8043892
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT159450.19822493887287398No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC158440.19696932778311793No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG95550.11878578180810981No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC86750.10784580399637389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT47550.025.9938531
GACTCTA54650.023.113684
CTAGCAG54250.022.831628
ACTCTAG55400.022.611295
CTGACTC57350.021.5403522
GCCGGGG11250.020.2305321
CTATCGA62900.019.80356417
CTCTAGC64950.019.3404876
TCTATCG65550.018.84235816
TAGCAGA67600.018.6850269
TGACTCT72700.017.471483
TAGCATG68300.017.31892827
CTAGCAT71500.016.54258226
ATCTATC76150.016.26536615
ATTCTAG74350.016.19066423
GATCTAT76750.016.13861314
GCATGAC75450.016.00288629
GCAGATC78850.015.93057111
TTCTAGC78550.015.2801324
AGCAGAT83050.015.25132410
Produced by FastQC (version 0.11.2)