FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9964050
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC258930.25986421184157044No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT196270.19697813640035927No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG171190.17180764849634436No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG123590.12403590909319001No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC120670.12110537381887887No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA116390.1168099317044776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT55000.029.4771821
ACTCTAG64400.025.59165
GACTCTA65200.025.009384
CTAGCAG66150.024.8596828
CTGACTC67750.024.2764852
CTCTAGC79250.020.9286636
CTATCGA77400.020.79472417
TGACTCT80650.020.3487363
TAGCAGA81500.020.3063149
TCTATCG79300.020.29628416
TAGCATG85350.018.65664327
GCGGGGG10500.018.3417631
CTAGCAT88050.018.08336826
ATTCTAG90800.017.72732223
GATCTAT92550.017.57984214
GCATGAC93650.017.07779729
ATCTATC95650.016.90000215
TTCTAGC96050.016.61272224
GCAGATC98950.016.44254511
AGCAGAT100950.016.1514510
Produced by FastQC (version 0.11.2)