FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9108694
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC232670.25543727783587855No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT173830.19083965275373177No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG149560.16419477918568787No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA126090.13842818739986215No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG108540.11916088080245094No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC100660.11050980524760191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT48000.027.6462961
CTAGCAG54000.024.3632358
ACTCTAG55350.023.7694025
GACTCTA58250.022.6461014
CTGACTC59400.022.0328832
TAGCAGA65400.020.1699339
CTATCGA65350.020.07850317
TCTATCG66950.019.59822816
CTCTAGC68950.019.5884956
TAGCATG70600.018.04430427
GCGGGGG9950.017.9467621
TGACTCT76850.017.347193
CTAGCAT75450.016.97586426
ATTCTAG78100.016.39931723
ATCTATC80950.016.38168115
GCGGGCT7450.015.9794441
GATCTAT80900.015.96000414
TATCGAT83700.015.84388718
GCATGAC80550.015.72864129
GCAGATC84000.015.57894111
Produced by FastQC (version 0.11.2)