FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15108512
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC307550.2035607477427294No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT289340.19150793936557087No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG211940.14027853967352974No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG184490.12210997350367793No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA166310.1100770214829892No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC154380.10218081039350534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT78950.030.5577161
CTAGCAG91900.026.1166428
ACTCTAG93750.025.827675
GACTCTA94650.025.7299564
CTGACTC98400.024.254532
CTATCGA108650.022.09132817
CTCTAGC117550.020.9853826
TAGCAGA116550.020.9232549
TCTATCG115900.020.52802316
TGACTCT125850.019.4624123
TAGCATG123550.019.17600427
CTAGCAT128600.018.36709826
GATCTAT136850.017.56458514
GCATGAC134650.017.54294429
ATTCTAG134750.017.50215723
ATCTATC137250.017.41119615
TTCTAGC138400.017.24283824
GCAGATC139900.017.18125511
TATCGAT141400.016.85131318
AGCAGAT149350.016.60945910
Produced by FastQC (version 0.11.2)