FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21686594
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC431820.19911840466972358No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT352240.1624229235812687No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG301190.13888303529821236No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG246280.11356324557005124No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA217680.10037537475917149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT104900.026.2718071
CTAGCAG120300.022.8898098
ACTCTAG122550.022.6423765
GACTCTA123000.022.559594
CTGACTC131200.020.9116082
CTATCGA142950.018.9942717
TCTATCG149150.018.181216
TAGCAGA154650.018.1223989
CTCTAGC157050.017.891216
TAGCATG155050.017.26648127
TGACTCT168650.016.660783
CTAGCAT161150.016.56843226
GCATGAC173900.015.45563429
ATTCTAG174850.015.44983323
GCGGGGG20250.015.2176751
GATCTAT180450.015.18280414
TTCTAGC183200.014.82215624
ATCTATC185300.014.80412515
GCAGATC190700.014.45824611
TCTAGCA259650.014.3107797
Produced by FastQC (version 0.11.2)