FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18070050
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC454960.2517757283460754No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG350600.19402270607994995No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT344770.19079637300394853No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG235350.13024313712468977No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA206550.11430516240962256No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC193880.10729356033879263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT98800.026.3659361
ACTCTAG113900.023.408955
CTAGCAG113150.023.3454518
GACTCTA116650.023.0970614
CTGACTC122350.021.2789882
TAGCAGA137500.019.3385169
CTCTAGC138950.019.2643176
CTATCGA136100.019.126517
TCTATCG141100.018.4734316
GCGGGGG17700.018.1987611
TGACTCT147800.018.0163573
TAGCATG150900.016.95279727
CTAGCAT156050.016.41433526
ATTCTAG159750.016.07703423
GCCGGGG20850.015.7851621
GATCTAT165900.015.77536914
GCATGAC163250.015.75657429
GCAGATC170250.015.41310211
ATCTATC170250.015.35161915
TTCTAGC168750.015.34425624
Produced by FastQC (version 0.11.2)