FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14231715
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC294240.20674950278304474No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT239300.1681455818922737No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG207830.14603299742863035No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG153720.10801228102164778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT68350.026.6407031
ACTCTAG81300.023.1552225
CTAGCAG81000.022.7212418
GACTCTA83400.022.4463124
CTGACTC85000.021.3674642
CTATCGA96000.019.20803317
TAGCAGA102700.018.3633179
CTCTAGC103550.018.1798136
TCTATCG101300.018.03017816
TAGCATG104250.017.4894827
TGACTCT115700.016.3315283
CTAGCAT111950.016.2545426
ATTCTAG116500.015.73897123
GCATGAC116150.015.6981729
GATCTAT120750.015.30006814
GCGGGGG14750.015.1938841
GCCGGGG16300.014.8232141
TTCTAGC124450.014.73354724
ATCTATC126700.014.49850315
GCAGATC129300.014.36922811
Produced by FastQC (version 0.11.2)