FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23941193
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT519070.21681041542081886No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC476290.19894163168894716No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG294890.1231726422321561No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG264640.11053751582053577No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC243890.10187044563735817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT132700.029.8172591
CTAGCAG152100.025.9944698
ACTCTAG154600.025.7790475
GACTCTA160400.024.8905664
CTGACTC167150.023.5734752
CTATCGA185250.021.45664617
CTCTAGC189950.021.1473126
TAGCAGA192300.020.8696849
TCTATCG193000.020.34093516
TGACTCT203500.019.6533743
TAGCATG201250.019.3196727
CTAGCAT209900.018.50555626
GCATGAC222350.017.58134529
ATTCTAG220900.017.55181523
GATCTAT230350.017.21013614
TTCTAGC229050.016.92697324
GCGGGGG20500.016.909921
ATCTATC234600.016.8085915
GCAGATC237250.016.7831411
TATCGAT242650.016.41005518
Produced by FastQC (version 0.11.2)