FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17951304
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC438520.244283089406764No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT327280.1823154462762148No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG289170.1610857907592674No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG252430.1406193109982428No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA225870.12582372846006062No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC183670.1023156869272561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT85650.029.269311
CTAGCAG101700.024.805248
ACTCTAG104800.024.2729645
GACTCTA107900.023.7702354
CTGACTC107250.023.492592
CTATCGA120900.020.54800417
TAGCAGA130550.019.7255949
TCTATCG127250.019.60489516
CTCTAGC133950.019.1735746
TGACTCT137500.018.7295463
TAGCATG133100.018.45806727
CTAGCAT141150.017.37932226
ATTCTAG146500.016.83911923
GCATGAC148650.016.76295929
GATCTAT152300.016.4034314
TATCGAT155250.016.11463518
ATCTATC160200.015.725306515
GCAGATC162550.015.67020811
TTCTAGC158000.015.61357424
CGCGGGG11150.015.386791
Produced by FastQC (version 0.11.2)