FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15810177
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC426330.2696554251100415No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT362220.22910559445349665No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG325250.20572192202528788No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG215310.1361844336088078No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC180940.1144452715488258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT91400.029.9962271
CTAGCAG102400.026.5488138
GACTCTA104650.026.2794914
ACTCTAG104200.026.2586675
CTGACTC110300.024.7774872
TAGCAGA126100.021.8365339
CTCTAGC129950.021.4054576
TGACTCT132900.020.9831713
CTATCGA128450.020.92035317
TCTATCG135050.020.07900216
TAGCATG144050.018.51271627
CTAGCAT150500.017.78783626
GATCTAT153300.017.68898214
ATTCTAG155050.017.24284223
ATCTATC160500.017.09133715
GCATGAC157900.017.08853329
GCAGATC162650.016.82228511
TATCGAT161900.016.6847318
TTCTAGC162350.016.661324
GCCGGGG18500.016.6556661
Produced by FastQC (version 0.11.2)