FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_hsfb2awchx2_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_hsfb2awchx2_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10468338
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC277640.26521879595404735No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT209720.2003374365634736No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG188740.18029605081532524No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG142680.13629670727101093No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA119880.11451674563813283No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC115420.11025627945906982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT55650.028.18611
CTAGCAG61400.025.6430428
ACTCTAG62200.025.4825925
GACTCTA66450.024.3267924
CTGACTC67600.023.5004432
CTATCGA73400.021.07007617
TAGCAGA76800.020.637759
CTCTAGC79100.020.4804866
TCTATCG78850.019.70230516
TAGCATG82050.018.81037527
CTAGCAT85450.018.2241826
TGACTCT87750.018.222633
GATCTAT90700.017.39847614
ATTCTAG89350.017.34956223
GCATGAC90400.017.15009329
GCGGGGG10100.016.9862231
TTCTAGC93900.016.50871724
ATCTATC95000.016.42649715
GCAGATC98400.016.2501311
TATCGAT95850.016.24478718
Produced by FastQC (version 0.11.2)