FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11862370
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT150300.12670317988732438No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT135960.11461453318350381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT47150.024.3604261
CTAGCAG55050.020.5298758
ACTCTAG60850.019.8965
GACTCTA61950.019.3732764
CTGACTC62000.018.569112
CTATCGA67000.016.45069317
TAGCAGA74200.015.8916499
CTTCCGA63250.015.7235491
TCTATCG71350.015.49652116
CTCTAGC80150.014.8867916
GCGGGCT11550.014.552981
TGACTCT83350.014.3153143
CGGGGGT7250.014.0072441
CTAGCAT79300.013.94478326
CCGATCT68600.013.8227824
TCTAGCA120200.013.6811227
GATCTAT83600.013.51911714
ATCTATC84150.013.18099215
GCGGGGG8550.013.1061941
TTCTAGC85800.013.05067824
Produced by FastQC (version 0.11.2)