FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10736327
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA46050.020.8312111
CCGATCT50900.018.7667714
TTCCGAT57400.016.9475922
TCCGATC65550.014.6260353
CTATCGA32850.013.73994217
TATGCCG11550.013.636682546
GCTGACT39000.012.9268121
CGATCTG31150.012.8053525
GACTCTA41750.012.7388974
TCTATCG35250.012.70519616
CTAGCAG40050.012.2306638
CGTATGC12300.011.95161144
ACTCTAG43550.011.5696185
TCTAGCA74750.010.3909717
CTCTAGC50700.010.3520886
TATCGAT43950.010.34958818
CGGGAAT56000.010.1279271
GTACCGA49000.010.0682416
CTGACTC51150.010.0564982
GCGGGAT15900.09.9085241
Produced by FastQC (version 0.11.2)