FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11943515
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT124860.10454208832157033No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT119660.10018826116097314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT46500.019.2779641
CTAGCAG50100.018.2970548
ACTCTAG55050.017.4792215
CTTCCGA69550.016.816031
GACTCTA59850.016.3699024
CGGGGGT9650.015.9661241
CCGATCT76950.015.1876314
TATGCCG20900.014.90482346
CGTATGC22050.014.60380344
CTATCGA61850.014.53851717
CTGACTC62700.014.3992792
GTATGCC23250.013.84936345
CTCTAGC73050.013.3159246
TCTATCG65350.013.17075816
TAGCAGA69050.012.971619
TCTAGCA113100.012.9305967
TTCCGAT97250.012.4861472
GCGGGGG8850.012.2657071
TGACTCT82000.011.7773463
CTAGCAT75250.011.76538726
Produced by FastQC (version 0.11.2)