FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13751787
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT143700.1044955102925896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT51350.021.9557421
CTTCCGA81950.019.9953751
CTAGCAG55850.019.8594428
CCGATCT89200.018.0836454
ACTCTAG67150.017.404055
CTGACTC66300.016.9424482
GACTCTA70900.016.6809334
CTATCGA69650.015.72402317
TTCCGAT114200.014.3405032
TCTATCG76050.014.21657416
TAGCAGA83100.013.894539
CTCTAGC86850.013.8592116
GCGGGCT12900.013.5710351
CTAGCAT80900.013.40948326
GCGGGGG9750.013.2870871
GCGGGGT13450.013.2764061
CGATCTG56200.013.1369965
CGGGGGG5450.012.84890651
TCTAGCA124050.012.7485737
GGACAGC83750.012.70559740
Produced by FastQC (version 0.11.2)