FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9279196
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT101830.10974011110445345No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT101820.10972933430870518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18900.024.07505846
CGTATGC20050.022.51962344
GTATGCC20900.021.26835445
GCTGACT39200.020.0080431
ACTCTAG43700.019.6178345
CTTCCGA54100.019.481031
CTAGCAG42250.018.1366928
CCAGGCG25200.017.77831333
CTGACTC45700.017.6126562
GACTCTA47850.017.5507534
TCGTATG25750.017.39923943
CCGATCT61600.016.7006384
CAGGCGA28050.016.0967434
CTATCGA48400.015.47158517
CTCGTAT27950.015.40338342
TTCCGAT69450.015.2176622
GCACACG38200.015.2052111
GCCGTCT30400.015.08358649
CCCTACA14250.014.97830710
TCTATCG50350.014.73307816
Produced by FastQC (version 0.11.2)