FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11864886
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT162330.13681547382756143No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT159310.13427014806547657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT56800.024.5372091
CTAGCAG59450.023.3640548
CTGACTC61750.022.838392
ACTCTAG62700.022.6571165
GACTCTA67300.021.524514
CGTATGC28900.021.19559544
TATGCCG29500.020.40810646
GTATGCC31600.019.0512345
CTTCCGA61350.019.0072861
CTATCGA74400.018.38764817
TCTATCG76200.018.04482316
TAGCAGA81050.017.482979
CTCTAGC84100.017.3910036
TCGTATG33900.017.3472143
TGACTCT87500.016.715733
CTCGTAT36450.016.2297942
CCGATCT72650.015.7492594
GCACACG64350.015.44146411
TATCGAT90200.015.32236318
ATCTATC93100.014.95713515
Produced by FastQC (version 0.11.2)