FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13989540
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT160230.11453557443632885No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT145630.10409920554928898No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC141950.10146866873392549TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36500.031.74221846
CGTATGC37750.031.1552144
GTATGCC39000.029.6171845
TCGTATG46150.025.25875743
CTCGTAT45950.024.91115842
GCTGACT54050.024.2285371
GCACACG65050.023.12802511
CTTCCGA87150.022.5396291
CTAGCAG58700.021.814488
TATCTCG53300.021.73833539
ACGTCTG70000.021.34259615
GCCGTCT56000.020.75185249
ACACGTC71950.020.7157613
CACACGT73250.020.53894812
ACGATAT58900.020.44208135
ACTCTAG68800.019.7321625
AGCACAC77250.019.6112510
GACTCTA72550.019.4838734
CCGATCT98850.019.3262394
CACGTCT79150.019.0524114
Produced by FastQC (version 0.11.2)