FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11993230
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT145880.12163528924234755No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT144290.12030954129954982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT48950.022.3181611
GTATGCC22550.021.26350645
CGTATGC22300.020.87435544
TATGCCG22600.020.5975246
CTAGCAG53800.019.9015058
ACTCTAG59700.019.4594465
GACTCTA61100.018.9562954
CTGACTC62400.017.8339392
CTTCCGA66450.016.704011
CTATCGA66950.016.3066717
GCGGGGT9050.015.8632351
CTCTAGC75500.015.4334846
TCTATCG70800.015.36993616
TAGCAGA72000.015.3568879
TCGTATG31950.015.2276843
GCGGGCT10650.015.1239321
CTCGTAT30150.014.743752542
TGACTCT81700.014.3908853
GCACACG43900.014.10785511
CCGATCT77250.013.9061394
Produced by FastQC (version 0.11.2)