FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13735451
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT139410.10149648526284286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT55500.021.6387331
CTAGCAG56500.021.3025078
ACTCTAG66850.019.2089125
GACTCTA68200.019.1878764
CTTCCGA82900.018.8792041
CTGACTC68400.017.7525392
CTATCGA68500.017.11154217
CCGATCT93050.016.5829544
TCTATCG74150.015.90166716
TAGCAGA76950.015.4593429
CTCTAGC87400.014.7324336
CTAGCAT78400.014.59570826
CCCTACA18900.013.8843810
TTCCGAT115450.013.8215912
TCTAGCA124550.013.76457
TGACTCT96150.013.5739783
GGACAGC85900.013.48817540
GCGGGCT16000.013.1299171
CGCGGGG7750.013.101681
TTCTAGC90750.013.03267324
Produced by FastQC (version 0.11.2)