FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14860954
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT176160.11853882328146632No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT164810.11090135936091318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT58700.021.175941
CTAGCAG64150.019.4162438
ACTCTAG69800.018.5470245
CTTCCGA88150.017.5968071
GACTCTA76400.017.127984
TATGCCG24850.017.0424946
GTATGCC23900.016.84089745
CGTATGC24550.016.82321244
CCGATCT95600.016.066994
GCGGGCT11800.016.023751
CTGACTC75750.015.9833622
CTATCGA78350.015.27236417
TCTATCG80750.014.81849916
TAGCAGA88700.014.3973019
CTCTAGC90700.014.1189276
CGCGGGG8700.013.68396951
CGGGGGG8500.013.5940061
TTCCGAT118600.013.1590592
TCTAGCA138350.013.0740487
TGACTCT100450.012.9576653
Produced by FastQC (version 0.11.2)