FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10710649
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT132710.12390472323385818No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT128180.11967528765063629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT47800.019.4863761
ACTCTAG51500.019.1595155
GACTCTA56350.017.7588484
CTTCCGA57850.017.7354261
CTAGCAG52550.017.5776868
CTGACTC55250.016.8475462
CCGATCT64500.015.5148994
TCTATCG64250.014.32257916
TAGCAGA67700.014.3159979
CTATCGA63550.014.31556717
CGGGGGT8400.013.7566271
CCCTACA15400.013.63217410
TTCCGAT74400.013.5929012
CTCTAGC73800.013.5597716
CGTATGC12850.013.34699944
TGACTCT76450.013.0901123
TCTAGCA115700.012.3983187
GCGGGAC10050.012.194931
CGCGGGG6050.012.1546171
CTAGCAT75750.012.10330326
Produced by FastQC (version 0.11.2)