FastQCFastQC Report
Thu 10 Nov 2016
HHC2KBGXY_n01_chxtest_g07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHC2KBGXY_n01_chxtest_g07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12913505
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT180900.14008590231699294No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT180360.1396677354444049No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGA147820.11446930945548865No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA144020.11152665368542468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT61000.024.3961281
CTAGCAG65850.022.9008678
ACTCTAG67250.022.7901695
CTGACTC70150.021.300982
GACTCTA75550.020.5184
CTTCCGA71700.019.5345151
TAGCAGA83250.017.9882939
TCTATCG81950.017.8045516
CGGGGGT10450.017.7591191
CTATCGA81750.017.67710917
CTCTAGC88300.017.594956
CCCTACA18000.017.30040710
CCGATCT84200.016.2906994
TGACTCT94400.015.9392273
TCCCTAC21850.015.0524379
TTCCGAT93750.014.9682992
CGTATGC18400.014.83725444
TCTAGCA147800.014.8192187
CTAGCAT98850.014.76228926
GCGGGGG9550.014.6662281
Produced by FastQC (version 0.11.2)