FastQCFastQC Report
Fri 30 Aug 2024
HH7YWDRX5_n02_16fluc_10X.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7YWDRX5_n02_16fluc_10X.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492966264
Sequences flagged as poor quality0
Sequence length90
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG15099730.3063035161367553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA5112380.1037064881178157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCA4966090.1007389422494031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)