FastQCFastQC Report
Fri 4 Nov 2016
HH7T5BGXY_n02_grads_set4_dna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7T5BGXY_n02_grads_set4_dna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113310514
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGC11301830.9974211219269555No Hit
TTGAGAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGG10446730.9219559272319602No Hit
TTGAGTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCA8428160.743810940615802No Hit
TTGAGTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCACATAC6829800.6027507738602262No Hit
TTGAGTAGCATCAGACGGAAAAAAACAGAATTATCGTATGGAATCTAAGA4146920.3659783945556897No Hit
TTGAGTATCACAGCCAAAGGCATTAGAAAGGCTTGAAATCCAAGGAAAGA3754070.33130817851554356No Hit
TTGAGTAGCATCAAACGGAATCATCGAATGGAATGGAATGGAATCATCAT3719770.32828109843363695No Hit
TTGAGTCCATTCGATGATGATTCCATTCCATTCCATTCGATGATTCCGTT3605640.31820877628354943No Hit
TTGAGTCCATCCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT3602880.3179651978279791No Hit
TTGAGTTTAAACTCCAAGTTAGATCAAACTAGAAAGACTTTTGAAGCAAA3457550.3051393800931836No Hit
TCAAGGCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGA3028360.26726204772136153No Hit
TGAGTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGCC2933140.2588585910041852No Hit
TGAGTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCAG2794680.2466390718164071No Hit
TGAGAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGGG2739620.24177985813390623No Hit
TTGAGTCCATTCTATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT2615140.23079411677543005No Hit
TTGAGTCCAACCAATGATTCCATTCATTTCCATTCAATGATTCCATTCGA2293390.20239869355812823No Hit
CAAGGCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGAC2206970.19477186380074138No Hit
TTGAGTCTAGCTGGTCGAGTAGATGAGGCGACAGAGTGAATAGAGCAAGG2158450.19048982515426593No Hit
TTGAGTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCTCATAC2138290.18871064339183918No Hit
TTGAGTATCACAGTAGTCCTCTGTGTACCTACTGGCCCAGAATGCAGGAG2041520.18017039442606358No Hit
TCAAGAATTAATTGAAGACGTTCTTCCTAAGAAAGCTAAAGATCAATTGA1998880.17640728379362924No Hit
TTGAGTTGGAACGCATCGAATGCACTCCAATGGAATCAACATCGAATGGA1903790.16801529997472256No Hit
TTCAGGTCCTTGCTGACAATCACCCGATTAGTAGCCACCCGTCGCTCATA1877860.16572689803525203No Hit
TTGAGTCCTAGCTGGGCACGAAATAATAACCTTCACGATCTACTCACACA1747550.15422664131591532No Hit
TTGAGTAGACGACTGTCCATCACTGCCGGGTGTAATCTTCGATGCGATCC1522110.1343308706551274No Hit
TTGAGTTACAGACCGAAGAAGAAGCAATAAAACCCTGAATCACATCAAGA1486770.13121200738706382No Hit
TTGAGTAGCATCATCATCTAATGGAATCGAATGGAATCATCGAATGGACT1460670.1289086024267792No Hit
TTGAGTCAGAGCGTGCCAGTACTTTGATTGCCAGGGAGTCTGTCCAAATA1415340.12490809105322742No Hit
TCCAGCCTGGGTGACACAGCAAGACCTTGTCTCCAAAAAATAATAAAATA1382330.12199485742338086No Hit
TGAGTAGCATCAGACGGAAAAAAACAGAATTATCGTATGGAATCTAAGAG1344710.11867477717028094No Hit
TGAGTCCATCCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATTC1307410.11538293789753702No Hit
TGAGTCCATTCGATGATGATTCCATTCCATTCCATTCGATGATTCCGTTT1259450.11115032096668453No Hit
CCAGCCTGGGTGACACAGCAAGACCTTGTCTCCAAAAAATAATAAAATAA1194570.10542446219950959No Hit
TGAGTAGCATCAAACGGAATCATCGAATGGAATGGAATGGAATCATCATC1178110.10397181677244884No Hit
TCAAGTTCATCTGCTGTGAGATATTGTGGCATTTCTTCTGTGTTATACTT1159440.10232413207480465No Hit
TTGAGTGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGG1157000.10210879459959028No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1141600.10074969741995876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA19992500.0136.446731
TTGAGTT23425450.0135.429141
TTGAGTC16002350.0134.290041
CGCCTTA1763400.0127.432563
GCCGCCT1663800.0123.866491
CCGCCTT1819950.0123.5451662
GCCTTAC1879500.0119.690974
TTGAGTG7777550.0116.6981661
TGAGTAT11465250.0106.449352
GAGTATC5479100.0105.993933
TGAGTCT7038750.0105.224982
AGTATCG2505150.0104.238424
TGAGTTC10234850.0103.861172
GAGTCCT4204150.0103.7136463
TACACTG2168650.0103.6691368
TATCGAG1486950.0103.265956
AGTCCTA2820900.0102.748394
TGAGTCC11596250.0102.2632
TGAGTTT8194300.0101.908582
CCTAGCT2676350.0101.7553257
Produced by FastQC (version 0.11.2)