FastQCFastQC Report
Fri 4 Nov 2016
HH7T5BGXY_n02_grads_set4_cdna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7T5BGXY_n02_grads_set4_cdna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105569978
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTT32923883.1186783045460142No Hit
TGAGTAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTTC17270751.6359527895326453No Hit
TTGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGG7470460.7076311032289881No Hit
TTGAGTGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTA5896700.5585584189474776No Hit
TTGAGTGATAAACTAATACTTTGTTATAATTTGATATTGTATCCACAAAA5481880.5192650509030133No Hit
TGGAGTGGAATGGAATGGAGTGGAGTGCAATGGAATGGAATGGAATGGAA4391530.41598284694158033No Hit
TTGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATTGAAG3165710.29986839629728823No Hit
TTGAGTATAAGCAGCCCTTATTCCTGAAGATTTGACAGGAAATCATATTT3080020.2917515053380043No Hit
TTGAGTATCACAGGTAGATTCTGGATATTAGCCTTTTGTCAGATGGATAG2985270.2827764158480738No Hit
TTAAGCCTAAAAATAAGGCAAAGGTAAGTATTTTACTAACTTCTAATTTT2701540.25590040380608964No Hit
TTGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAAG2286090.21654735970485853No Hit
TGAGTGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTAC2258670.21395003037700736No Hit
TTGAGTATTACAGCCATTTGATCGTTGACAAACCTGACAAAAACAAGCAA2225910.2108468754251327No Hit
TTGAGTTGACTACCACACGTCACAAACCAGTTTCTGAGAATGCTTCTGTC2205560.20891924406766477No Hit
TTGAGTTCGTCAAACCGCTTAAAAAAGATTAGGGATGATGCTGAATCTAA2143920.20308046289447937No Hit
TTGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATTGAGG2051490.1943251328516901No Hit
TTGAGTTTGCGAGAGGGTTAAGAGGAATCTTGTTGTTAATGCTCAGGATA2008100.19021506284674985No Hit
TTGAGTATCACAGGGGAACCAGGGACGTAAGGCAGACGCCACAGCCCTGA1935920.18337789177146557No Hit
TTGAGTATGCCAGAGTTAGAAGTATCTTTTATCTCAGCAGTCTTAGCTAA1922510.18210764427742895No Hit
TTGAGTATCACAGGGAGACCAAGAAACTACCACATACAACATGTCCTGAA1919310.18180452779861336No Hit
TTGAGTCCTCATTTGATTTGGCTCTTTTCAACTTTTGTTTCCTAATCTTA1903450.18030220675048356No Hit
TTGAGTTAATTTTTGTATAAGGTGTAAGGAAGTGGTCCAGTTTCAGTTTT1794080.16994225384796424No Hit
TTGAGTTGCACTAGACAGAAGCATTCTCAGAAACTTATTTGTGAAGTGTG1750150.16578103293722388No Hit
TTGAGTATCACAGGCCTGATTGACCTGGATGATTCTATTAAATACATAAA1682050.15933033537242947No Hit
TTGAGTGTATGTGTGTGTGTTTTCTGTAGCATATGTTTATTTCTTCTTGT1519530.14393580720458235No Hit
TGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGGG1294680.1226371383728052No Hit
TCAAGATGGATTAAAGACTTACATCTTTGACCTAAAACCATAAAAACCCT1127270.106779410335768No Hit
TCAAGGAGGAGCTAGATAGGGAATATGTTGGGTTTCCCTGAATAAATCAG1105380.10470590417287005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA26021050.0137.296231
TTGAGTT23459750.0133.204681
TTGAGTC10340050.0131.56471
TTGAGTG13637900.0123.622821
GAGTATC4808650.0115.3469853
AGTATCG1614700.0112.233354
TGAGTTC6452550.0110.078322
GAGTCCT3209700.0110.0735863
TGAGTAT12601900.0109.301432
TGAGTTA12391950.0108.788022
TGAGTAC5614700.0108.550532
GTATCGC780800.0108.394675
CTTCCGA1084300.0108.325357
TGAGTCC6106150.0107.9052052
CCGCCTT735650.0107.756352
GAGTTAC3803550.0107.58693
CGCCTTA740650.0106.883923
GAGTTCC2226900.0106.743293
TGAGTCT4556100.0106.53072
TCTTCCG1111300.0106.049466
Produced by FastQC (version 0.11.2)