FastQCFastQC Report
Fri 4 Nov 2016
HH7T5BGXY_n01_grads_set4_dna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7T5BGXY_n01_grads_set4_dna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113310514
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8203350.72397076938509No Hit
TTGAGAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGG7409710.6539296079797149No Hit
CAAGGCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGAC6749900.5956993540775924No Hit
TTGAGTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGC6305560.5564849877920419No Hit
GCAAGGCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGA4751220.4193097208966857No Hit
TTGAGTCCTAGCTCCATACACTCTGTCGCCACATCTACTCGACCTCTGAC3912700.3453077619963846No Hit
TTGAGTAGCATCAGACGGAAAAAAACAGAATTATCGTATGGAATCTAAGA3883600.34273959784526264No Hit
CCAGCCTGGGTGACACAGCAAGACCTTGTCTCCAAAAAATAATAAAATAA3635860.32087578386591736No Hit
TGAGAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGGG3271190.28869253915836973No Hit
GCAAGTTCATCTGCTGTGAGATATTGTGGCATTTCTTCTGTGTTATACTT3057690.2698505100771143No Hit
TCAAGGCTGCAGTGAGCCATGATGGCGCCACTGTACTCCAGCCTGGGTGA3037380.26805809035514566No Hit
TTGAGTAGCATCAAACGGAATCATCGAATGGAATGGAATGGAATCATCAT3017510.26630450198116656No Hit
TTGAGTCCATCCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT2893580.2553672998076772No Hit
TTGAGTCCATTCGATGATGATTCCATTCCATTCCATTCGATGATTCCGTT2646520.23356349791158834No Hit
TCAAGAATTAATTGAAGACGTTCTTCCTAAGAAAGCTAAAGATCAATTGA2530010.22328113347010325No Hit
TTCAGGTCCTTGCTGACAATCACCCGATTAGTAGCCACCCGTCGCTCATA2503550.2209459574069181No Hit
CAAGTTCATCTGCTGTGAGATATTGTGGCATTTCTTCTGTGTTATACTTC2307820.2036721852660557No Hit
TGAGTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGCC2201590.19429706231850646No Hit
GTGAGTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCA2139070.18877948078145687No Hit
TTGAGTCCATTCTATGATTGTTCCATTCGATTCTATTCGGTGATTCCATT2056270.18147212711434704No Hit
GTGAGAGTGTTACAGCGACGAGAGAAGGTAGCTCAGCTGTTCAGGGAGGG2008760.17727922406212013No Hit
TGAGTCCATTCGATGATGATTCCATTCCATTCCATTCGATGATTCCGTTT1937030.17094883180919998No Hit
GCCAGCCTGGGTGACACAGCAAGACCTTGTCTCCAAAAAATAATAAAATA1900070.16768699857808428No Hit
GTGAGTAGTTCTACCTGTCTGTTGATACCCTCCTGATAGCCTGCCACCGC1847780.16307224588178992No Hit
TGAGTAGCATCAAACGGAATCATCGAATGGAATGGAATGGAATCATCATC1846080.16292221567364878No Hit
TTGAGTTGGAACGCATCGAATGCACTCCAATGGAATCAACATCGAATGGA1742150.15375007477240815No Hit
TGAGTAGCATCAGACGGAAAAAAACAGAATTATCGTATGGAATCTAAGAG1740130.15357180358391104No Hit
TGAAGTGGATCAGGGGAGTGTGGCGAGAGACGGCGGTGGCAGGCTATCAG1709370.15085713934719244No Hit
TTGAGTAGCATCATCATCTAATGGAATCGAATGGAATCATCGAATGGACT1682880.14851931569209897No Hit
CAAGAATTAATTGAAGACGTTCTTCCTAAGAAAGCTAAAGATCAATTGAA1665780.14701018830432627No Hit
TTGAGTCCAACCAATGATTCCATTCATTTCCATTCAATGATTCCATTCGA1644660.14514628360083162No Hit
TTGAGTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCA1544970.1363483356893077No Hit
TGAGTCCATTCTATGATTGTTCCATTCGATTCTATTCGGTGATTCCATTC1501870.13254462864761163No Hit
TGAGTCCATCCGATGATTGTTCCATTCGATTCTATTCGGTGATTCCATTC1490920.13157825760105546No Hit
TTGAGTTACAGACCGAAGAAGAAGCAATAAAACCCTGAATCACATCAAGA1408380.12429384973048484No Hit
TCAGGTCCTTGCTGACAATCACCCGATTAGTAGCCACCCGTCGCTCATAC1309730.11558768500511789No Hit
TTGAGTTTAAACTCCAAGTTAGATCAAACTAGAAAGACTTTTGAAGCAAA1297940.11454718138512725No Hit
TCAAGTTCATCTGCTGTGAGATATTGTGGCATTTCTTCTGTGTTATACTT1238210.10927582589555634No Hit
TCCAGCCTGGGTGACACAGCAAGACCTTGTCTCCAAAAAATAATAAAATA1231090.1086474640826358No Hit
GGGAGTATACCGTACAGCAGTGTTGTCCAGTAGAGCTTTGTTTTAGAGCA1204730.10632111332581193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCCT2796950.0134.869661
CGCCTTA2830200.0133.695633
CCGCCTT2846000.0132.93342
TTGAGTA11213950.0131.653091
TTGAGTT14875300.0129.755691
TTGAGTC10788150.0129.38071
GCCTTAC3017600.0126.043154
GTGAGTA3376100.0123.2460251
TACACTG3392700.0111.208918
CCTTACA3423400.0111.0014345
GTGAGTC1982650.0109.8228151
CTTACAC3489950.0107.733126
GGGAGTA1930250.0107.063571
ACACTGA3580700.0106.831559
TGAGTAT7170900.0105.844692
GTGAGTT4526750.0104.102221
TTGAGTG5316100.0102.904891
GAGTATC4306200.0102.887863
GTCCTAG2492850.0101.613795
GGGAGTT2283950.0101.2585451
Produced by FastQC (version 0.11.2)