FastQCFastQC Report
Fri 4 Nov 2016
HH7T5BGXY_n01_grads_set4_cdna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7T5BGXY_n01_grads_set4_cdna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105569978
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTT26191742.4809837508917543No Hit
TGAGTAGGTTCCCTCCACTCTACTCTACTGAAATCGACTCCATTCCGTTC21783962.0634616405811887No Hit
TTGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGG9630800.9122669325553899No Hit
TGGAGTGGAATGGAATGGAGTGGAGTGCAATGGAATGGAATGGAATGGAA5750980.5447552522934125No Hit
TTGAGTGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTA5464920.5176585335652907No Hit
TTAAGCCTAAAAATAAGGCAAAGGTAAGTATTTTACTAACTTCTAATTTT4647720.4402501627877577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4604340.43614104002181375No Hit
TGAGTGAGTTGCGGTACTACTACTACTACTACTACTACTACTACTACTAC4542780.43030983676059875No Hit
TGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATGGAGGG2873960.27223269858027255No Hit
TTGAGTGATAAACTAATACTTTGTTATAATTTGATATTGTATCCACAAAA2533030.23993847947945957No Hit
CAAGGAGGAGCTAGATAGGGAATATGTTGGGTTTCCCTGAATAAATCAGT2323400.22008150840004911No Hit
TCAAGATGGATTAAAGACTTACATCTTTGACCTAAAACCATAAAAACCCT2227880.21103348150740353No Hit
TTGAGTTGACTACCACACGTCACAAACCAGTTTCTGAGAATGCTTCTGTC2078730.19690541187760785No Hit
TTAAGCTGGATCTTTTTTTGGGTAGCAGCCAGTTTAACTTGATGATAAAC1918660.18174295726385395No Hit
TTGAGTATTACAGCCATTTGATCGTTGACAAACCTGACAAAAACAAGCAA1857440.17594396012851304No Hit
TTGAGTTAATTTTTGTATAAGGTGTAAGGAAGTGGTCCAGTTTCAGTTTT1709470.16192766470028058No Hit
TCAAGGAGGAGCTAGATAGGGAATATGTTGGGTTTCCCTGAATAAATCAG1651330.15642041717579974No Hit
TAAGCCTAAAAATAAGGCAAAGGTAAGTATTTTACTAACTTCTAATTTTA1529260.14485747074798103No Hit
TTGAGTCCTCATTTGATTTGGCTCTTTTCAACTTTTGTTTCCTAATCTTA1521200.14409399611696425No Hit
TTAAGATGTGATTTCAATGCCAATGCAGTTTCTAAAAACAAATGTTGCTT1408290.1333987206097552No Hit
TTGAGTATCACAGGGAGACCAAGAAACTACCACATACAACATGTCCTGAA1311600.12423986675454265No Hit
TTGAGTGGAGTGGAGTGGAGTGGAATGTAGTGAAATGAAATGAATTGAGG1222750.11582364827242837No Hit
TTGAGTATCACAGGTAGATTCTGGATATTAGCCTTTTGTCAGATGGATAG1189960.1127176516035648No Hit
CAAGATGGATTAAAGACTTACATCTTTGACCTAAAACCATAAAAACCCTA1170840.1109065306426416No Hit
TTGAGTATCACAGGCCTGATTGACCTGGATGATTCTATTAAATACATAAA1126650.10672068151799748No Hit
TCCAGTCTACCGCACTCTACTTCATTCCATTCCACTCCTACTCCATTCCA1119610.10605382526460316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGTA16310500.0133.930761
GTGAGTA4457200.0129.75421
TTGAGTT14925100.0127.843481
TTGAGTC6443150.0123.595531
CCGCCTT1231650.0120.5903242
CGCCTTA1242450.0119.5122453
GCCGCCT1252450.0118.4261861
GTGAGTC2233200.0115.9674151
GAGTATC4035600.0115.527843
AGTATCG1475050.0113.279594
TTGAGTG9819000.0111.737971
TGAGTAT9526450.0110.5715562
GAGTCCT2725100.0110.019053
GTATCGC717450.0109.5729755
TGAGTTC5184500.0109.405292
TGAGTTA9943200.0108.219692
TGAGTAC4410300.0106.93292
GAGTTCC1991900.0106.177143
GAGTTAC3341300.0106.1750953
TGAGTCT3608300.0104.352672
Produced by FastQC (version 0.11.2)